{"id":113,"date":"2011-02-07T16:58:00","date_gmt":"2011-02-07T16:58:00","guid":{"rendered":"http:\/\/lucasgferreira.wordpress.com\/2011\/02\/07\/resistance-exercise-volume-affects-myofibrillar-protein-synthesis-and-anabolic-signalling-molecule-phosphorylation-in-young-men"},"modified":"2011-02-07T16:58:00","modified_gmt":"2011-02-07T16:58:00","slug":"resistance-exercise-volume-affects-myofibrillar-protein-synthesis-and-anabolic-signalling-molecule-phosphorylation-in-young-men","status":"publish","type":"post","link":"https:\/\/blog.ufes.br\/lucasgf\/?p=113","title":{"rendered":"Resistance exercise volume affects myofibrillar protein synthesis and anabolic signalling molecule phosphorylation in young men."},"content":{"rendered":"
J Physiol.<\/a> 2010 Aug 15;588(Pt 16):3119-30. Epub 2010 Jun 25.<\/div>\n

Burd NA<\/a>, Holwerda AM<\/a>, Selby KC<\/a>, West DW<\/a>, Staples AW<\/a>, Cain NE<\/a>, Cashaback JG<\/a>, Potvin JR<\/a>, Baker SK<\/a>, Phillips SM<\/a>.<\/span><\/h1>\n
Exercise Metabolism Research Group, Department of Kinesiology, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada.<\/div>\n
\n

Abstract<\/h3>\n
We aimed to determine if any mechanistic differences exist between a single set (1SET) and multiple sets (i.e. 3 sets; 3SET) of resistance exercise by utilizing a primed constant infusion of [ring-13C6]phenylalanine to determine myofibrillar protein synthesis (MPS) and Western blot analysis to examine anabolic signalling molecule phosphorylation following an acute bout of resistance exercise. Eight resistance-trained men (24+\/-5 years, BMI=25+\/-4 kg m2) were randomly assigned to perform unilateral leg extension exercise at 70% concentric one repetition maximum (1RM) until volitional fatigue for 1SET or 3SET. Biopsies from the vastus lateralis were taken in the fasted state (Fast) and fed state (Fed; 20 g of whey protein isolate) at rest, 5 h Fed, 24 h Fast and 29 h Fed post-exercise. Fed-state MPS was transiently elevated above rest at 5 h for 1SET (2.3-fold) and returned to resting levels by 29 h post-exercise. However, the exercise induced increase in MPS following 3SET was superior in amplitude and duration as compared to 1SET at both 5 h (3.1-fold above rest) and 29 h post-exercise (2.3-fold above rest). Phosphorylation of 70 kDa S6 protein kinase (p70S6K) demonstrated a coordinated increase with MPS at 5 h and 29 h post-exercise such that the extent of p70S6K phosphorylation was related to the MPS response (r=0.338, P=0.033). Phosphorylation of 90 kDa ribosomal S6 protein kinase (p90RSK) and ribosomal protein S6 (rps6) was similar for 1SET and 3SET at 24 h Fast and 29 h Fed, respectively. However, 3SET induced a greater activation of eukaryotic translation initiation factor 2B (eIF2B) and rpS6 at 5 h Fed. These data suggest that 3SET of resistance exercise is more anabolic than 1SET and may lead to greater increases in myofibrillar protein accretion over time.<\/div>\n
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<\/div>\n
Figure 3.\u2002Myofibrillar protein fractional synthesis rate (FSR) at rest and 5 h after protein ingestion after one set (1SET) or 3 sets (3SET) of resistance exercise and 24\u201329 h later<\/strong> Inset, volume load (kg times repetitions) performed during 1SET and 3SET of resistance exercise. *Significantly different from rest (P<\/em> < 0.05). \u2020Significantly different from 29 h (P<\/em> < 0.05). \u2021Significantly different from 1SET (P<\/em> < 0.05).<\/span><\/span><\/div>\n

<\/span><\/div>\n

<\/span><\/div>\n
<\/span><\/div>\n
Figure 4.\u2002Phosphorylation of eIF2B\u03b5Ser539<\/sup> (A<\/em>), p90RSKThr573<\/sup> (B<\/em>), p70S6KThr389<\/sup> (C<\/em>) and rps6Ser240\/244<\/sup> (D<\/em>) following one set (1SET) or 3 sets (3SET) of resistance exercise in the fasted or fed states<\/strong>Data are expressed as fold-change from rest. *Significantly different from Fast (P<\/em> < 0.05). \u2020Significantly different from 1 set (1SET) within that time point (P<\/em> < 0.05).<\/span><\/span><\/div>\n<\/div>\n
<\/div>\n","protected":false},"excerpt":{"rendered":"

J Physiol. 2010 Aug 15;588(Pt 16):3119-30. Epub 2010 Jun 25. Burd NA, Holwerda AM, Selby KC, West DW, Staples AW, Cain NE, Cashaback JG, Potvin JR, Baker SK, Phillips SM. Exercise Metabolism Research Group, Department of Kinesiology, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada. Abstract We … Continue lendo →<\/span><\/a><\/p>\n","protected":false},"author":9,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"_uag_custom_page_level_css":"","footnotes":""},"categories":[6,21,34,41],"tags":[],"class_list":["post-113","post","type-post","status-publish","format-standard","hentry","category-bioquimica","category-fisiologia","category-paper","category-treinamento"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false},"uagb_author_info":{"display_name":"Lucas Guimar\u00e3es Ferreira","author_link":"https:\/\/blog.ufes.br\/lucasgf\/?author=9"},"uagb_comment_info":777,"uagb_excerpt":"J Physiol. 2010 Aug 15;588(Pt 16):3119-30. Epub 2010 Jun 25. Burd NA, Holwerda AM, Selby KC, West DW, Staples AW, Cain NE, Cashaback JG, Potvin JR, Baker SK, Phillips SM. Exercise Metabolism Research Group, Department of Kinesiology, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada. Abstract We … Continue lendo →","_links":{"self":[{"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/posts\/113","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=113"}],"version-history":[{"count":0,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/posts\/113\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=113"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=113"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=113"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}