{"id":154,"date":"2011-07-21T21:36:00","date_gmt":"2011-07-21T21:36:00","guid":{"rendered":"http:\/\/lucasgferreira.wordpress.com\/2011\/07\/21\/controle-de-qualidade-no-pcr-em-tempo-real"},"modified":"2011-07-21T21:36:00","modified_gmt":"2011-07-21T21:36:00","slug":"controle-de-qualidade-no-pcr-em-tempo-real","status":"publish","type":"post","link":"https:\/\/blog.ufes.br\/lucasgf\/?p=154","title":{"rendered":"Controle de Qualidade no PCR em Tempo Real"},"content":{"rendered":"<div style=\"border-collapse:collapse;font-family:'Arial Narrow';text-align:center;margin:0\"><span class=\"yui_3_2_0_6_131127872450762\" style=\"font-family:Arial\"><b><span class=\"Apple-style-span\" style=\"font-size:large\">QUALITY CONTROL &#8211; EFFICIENCY CURVES<\/span><\/b><b><span class=\"Apple-style-span\" style=\"font-size:large\">&nbsp;<\/span><\/b><\/span><\/div>\n<div style=\"border-collapse:collapse;font-family:'Arial Narrow';text-align:center;margin:0\"><span class=\"yui_3_2_0_6_131127872450762\" style=\"font-family:Arial\"><br \/><\/span><\/div>\n<div align=\"center\" style=\"border-collapse:collapse;font-family:'Arial Narrow';margin:0\"><span>Here is a list of the criteria we apply in the lab before we accept the data for efficiency from a dilution curve<\/span><\/div>\n<ul style=\"border-collapse:collapse;font-family:'Arial Narrow';font-size:16px\">\n<li><span style=\"font-family:'Arial Narrow'\"><span class=\"Apple-style-span\" style=\"font-size:small\">\u2022<\/span><span class=\"Apple-style-span\" style=\"font-size:small\">use PCR baseline subtraction (not curve fitting default option)<\/span><\/span><\/li>\n<li><span style=\"font-family:'Arial Narrow'\"><span class=\"Apple-style-span\" style=\"font-size:small\">\u2022<\/span><span class=\"Apple-style-span\" style=\"font-size:small\">set the threshold manually to lab standard<\/span><\/span><\/li>\n<li><span style=\"font-family:'Arial Narrow'\"><span class=\"Apple-style-span\" style=\"font-size:small\">\u2022<\/span><span class=\"Apple-style-span\" style=\"font-size:small\">check all melting curves are OK<\/span><\/span><\/li>\n<li><span style=\"font-family:'Arial Narrow'\"><span class=\"Apple-style-span\" style=\"font-size:small\">\u2022<\/span><span class=\"Apple-style-span\" style=\"font-size:small\">check slopes are parallel in log view<\/span><\/span><\/li>\n<li><span style=\"font-family:'Arial Narrow'\"><span class=\"Apple-style-span\" style=\"font-size:small\">\u2022<\/span><span class=\"Apple-style-span\" style=\"font-size:small\">delete samples if multiple dilutions cross line together (usually at dilute end of curve)<\/span><\/span><\/li>\n<li><span style=\"font-family:'Arial Narrow'\"><span class=\"Apple-style-span\" style=\"font-size:small\">\u2022<\/span><span class=\"Apple-style-span\" style=\"font-size:small\">delete samples if can detect amplification at cycle 10 or earlier<\/span><\/span><\/li>\n<li><span style=\"font-family:'Arial Narrow'\"><span class=\"Apple-style-span\" style=\"font-size:small\">\u2022<\/span><span class=\"Apple-style-span\" style=\"font-size:small\">make sure there are 5 or more points<\/span><\/span><\/li>\n<li><span style=\"font-family:'Arial Narrow'\"><span class=\"Apple-style-span\" style=\"font-size:small\">\u2022<\/span><span class=\"Apple-style-span\" style=\"font-size:small\">check that correlation coefficient is more than 0.990<\/span><\/span><\/li>\n<\/ul>\n<div class=\"blogger-post-footer\"><img width='1' height='1' src='' alt='' \/><\/div>\n","protected":false},"excerpt":{"rendered":"<p>QUALITY CONTROL &#8211; EFFICIENCY CURVES&nbsp; Here is a list of the criteria we apply in the lab before we accept the data for efficiency from a dilution curve \u2022use PCR baseline subtraction (not curve fitting default option) \u2022set the threshold &hellip; <a href=\"https:\/\/blog.ufes.br\/lucasgf\/?p=154\">Continue lendo <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":9,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"_uag_custom_page_level_css":"","footnotes":""},"categories":[37],"tags":[],"class_list":["post-154","post","type-post","status-publish","format-standard","hentry","category-protocolos"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false},"uagb_author_info":{"display_name":"Lucas Guimar\u00e3es Ferreira","author_link":"https:\/\/blog.ufes.br\/lucasgf\/?author=9"},"uagb_comment_info":3,"uagb_excerpt":"QUALITY CONTROL &#8211; EFFICIENCY CURVES&nbsp; Here is a list of the criteria we apply in the lab before we accept the data for efficiency from a dilution curve \u2022use PCR baseline subtraction (not curve fitting default option) \u2022set the threshold &hellip; Continue lendo &rarr;","_links":{"self":[{"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/posts\/154","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=154"}],"version-history":[{"count":0,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/posts\/154\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=154"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=154"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=154"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}