{"id":375,"date":"2012-04-21T15:53:05","date_gmt":"2012-04-21T17:53:05","guid":{"rendered":"http:\/\/blog.ufes.br\/lucasgf\/?p=375"},"modified":"2012-05-03T13:55:06","modified_gmt":"2012-05-03T15:55:06","slug":"suplementacao-com-creatina-reduz-o-conteudo-de-especies-reativas-de-oxigenio-sem-alterar-a-expressao-e-atividade-das-enzimas-antioxidantes-no-musculo-esqueletico","status":"publish","type":"post","link":"https:\/\/blog.ufes.br\/lucasgf\/?p=375","title":{"rendered":"Suplementa\u00e7\u00e3o com creatina reduz o conte\u00fado de esp\u00e9cies reativas de oxig\u00eanio sem alterar a express\u00e3o e atividade das enzimas antioxidantes no m\u00fasculo esquel\u00e9tico"},"content":{"rendered":"
Eur J Appl Physiol.<\/a>\u00a02012 Mar 14. [Epub ahead of print]<\/div>\n

Short-term creatine supplementation decreases reactive oxygen species content with no changes in expression and activity of antioxidant enzymes in skeletal muscle.<\/h1>\n
Guimar\u00e3es-Ferreira L<\/a><\/strong>,\u00a0Pinheiro CH<\/a>,\u00a0Gerlinger-Romero F<\/a>,\u00a0Vitzel KF<\/a>,\u00a0Nachbar RT<\/a>,\u00a0Curi R<\/a>,\u00a0Nunes MT<\/a>.<\/div>\n
\n

Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of S\u00e3o Paulo, ICB-I, Cidade Universit\u00e1ria, Av. Prof. Lineu Prestes, 1524, Butant\u00e3, S\u00e3o Paulo, SP, 05508-900, Brazil, lucas@cefd.ufes.br.<\/p>\n<\/div>\n

\n

Abstract<\/h3>\n

The effect of short-term creatine (Cr) supplementation upon content of skeletal muscle-derived-reactive oxygen species (ROS) was investigated. Wistar rats were supplemented with Cr (5\u00a0g\/kg BW) or vehicle, by gavage, for 6\u00a0days. Soleus and extensor digitorum longus (EDL) muscles were removed and incubated for evaluation of ROS content using Amplex-UltraRed reagent. The analysis of expression and activity of antioxidant enzymes (superoxide dismutase 1 and 2, catalase and glutathione peroxidase) were performed. Direct scavenger action of Cr on superoxide radical and hydrogen peroxide was also investigated. Short-term Cr supplementation attenuated ROS content in both soleus and EDL muscles (by 41 and 33.7%, respectively). Cr supplementation did not change expression and activity of antioxidant enzymes. Basal TBARS content was not altered by Cr supplementation. In cell-free experiments, Cr showed a scavenger effect on superoxide radical in concentrations of 20 and 40\u00a0mM, but not on hydrogen peroxide. These results indicate that Cr supplementation decreases ROS content in skeletal muscle possibly due to a direct action of Cr molecule on superoxide radical.<\/p>\n<\/div>\n

\n
\n
\n
PMID:\u00a022415102<\/span><\/dt>\n<\/dl>\n
\u00a0<\/dl>\n<\/div>\n<\/div>\n

Nosso estudo recentemente publicado no European Journal of Applied Physiology traz novos dados que apontam para poss\u00edveis efeitos terap\u00eauticos da suplementa\u00e7\u00e3o com creatina, especialmente em doen\u00e7as neuromusculares.<\/p>\n

Doen\u00e7as como diabetes, distrofia muscular, DPOC, insufici\u00eancia card\u00edaca e outras t\u00eam em comum uma produ\u00e7\u00e3o exacerbada de esp\u00e9cies reativas de oxig\u00eanio (ROS). Em nosso estudo, mostramos que ap\u00f3s 6 dias de suplementa\u00e7\u00e3o com creatina monohidratada, o conte\u00fado de ROS no m\u00fasculo esquel\u00e9tico foi significativamente menor nos m\u00fasculos s\u00f3leo e EDL.<\/p>\n

\"\"<\/p>\n

A express\u00e3o g\u00eanica e atividade das enzimas antioxidantes n\u00e3o foi alterada. Entretanto, em experimentos\u00a0cell free<\/em>\u00a0demonstramos que a creatina pode atuar como scavenger contra o \u00e2nion super\u00f3xido, mas n\u00e3o o per\u00f3xido de hidrog\u00eanio.<\/p>\n

\"\"<\/p>\n

Concluindo, nossos dados suportam a a\u00e7\u00e3o antioxidante da creatina, demonstrando pela primeira vez uma redu\u00e7\u00e3o de ROS no m\u00fasculo esquel\u00e9tico\u00a0in vivo<\/em>. Esta a\u00e7\u00e3o parece envolver uma a\u00e7\u00e3o direta da mol\u00e9cula de creatina sobre ROS.<\/p>\n

Download do trabalho completo<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Eur J Appl Physiol.\u00a02012 Mar 14. [Epub ahead of print] Short-term creatine supplementation decreases reactive oxygen species content with no changes in expression and activity of antioxidant enzymes in skeletal muscle. Guimar\u00e3es-Ferreira L,\u00a0Pinheiro CH,\u00a0Gerlinger-Romero F,\u00a0Vitzel KF,\u00a0Nachbar RT,\u00a0Curi R,\u00a0Nunes MT. Department … Continue lendo →<\/span><\/a><\/p>\n","protected":false},"author":9,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"_uag_custom_page_level_css":"","footnotes":""},"categories":[6,11,21,27,31,34,40],"tags":[],"class_list":["post-375","post","type-post","status-publish","format-standard","hentry","category-bioquimica","category-creatina","category-fisiologia","category-musculo","category-nutricao","category-paper","category-suplementacao"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false},"uagb_author_info":{"display_name":"Lucas Guimar\u00e3es Ferreira","author_link":"https:\/\/blog.ufes.br\/lucasgf\/?author=9"},"uagb_comment_info":21,"uagb_excerpt":"Eur J Appl Physiol.\u00a02012 Mar 14. [Epub ahead of print] Short-term creatine supplementation decreases reactive oxygen species content with no changes in expression and activity of antioxidant enzymes in skeletal muscle. Guimar\u00e3es-Ferreira L,\u00a0Pinheiro CH,\u00a0Gerlinger-Romero F,\u00a0Vitzel KF,\u00a0Nachbar RT,\u00a0Curi R,\u00a0Nunes MT. Department … Continue lendo →","_links":{"self":[{"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/posts\/375","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=375"}],"version-history":[{"count":6,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/posts\/375\/revisions"}],"predecessor-version":[{"id":399,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/posts\/375\/revisions\/399"}],"wp:attachment":[{"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=375"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=375"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=375"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}