{"id":94,"date":"2010-12-06T20:25:00","date_gmt":"2010-12-06T20:25:00","guid":{"rendered":"http:\/\/lucasgferreira.wordpress.com\/2010\/12\/06\/novas-evidencias-para-o-papel-do-complexo-mtorc2"},"modified":"2010-12-06T20:25:00","modified_gmt":"2010-12-06T20:25:00","slug":"novas-evidencias-para-o-papel-do-complexo-mtorc2","status":"publish","type":"post","link":"https:\/\/blog.ufes.br\/lucasgf\/?p=94","title":{"rendered":"Novas evid\u00eancias para o papel do complexo mTORC2."},"content":{"rendered":"
Muito j\u00e1 foi publicado acerca da fun\u00e7\u00e3o do complexo mTORC1 em diversos tecidos. o mTORC2, em contrapartida, tem sua fun\u00e7\u00e3o ainda pouco conhecida. Kumar e colaboradores, neste trabalho, trazem novas evid\u00eancias \u00e0 tona, e auxiliam a desvendar a complexidade da via de sinaliza\u00e7\u00e3o da via da mTOR.<\/span><\/div>\n

<\/span><\/span><\/div>\n
Fat cell-specific ablation of rictor in mice impairs insulin-regulated fat cell and whole-body glucose and lipid metabolism.<\/span><\/span><\/div>\n
Diabetes.<\/a> 2010 Jun;59(6):1397-406. Epub 2010 Mar 23.<\/span><\/div>\n
Kumar A<\/a>, Lawrence JC Jr<\/a>, Jung DY<\/a>, Ko HJ<\/a>, Keller SR<\/a>, Kim JK<\/a>, Magnuson MA<\/a>, Harris TE<\/a>.<\/div>\n
Department of Pharmacology, University of Virginia Health System, Charlottesville, Virginia, USA. al4p@virginia.edu<\/div>\n
\n

Abstract<\/h3>\n
OBJECTIVE: <\/span>Rictor is an essential component of mammalian target of rapamycin (mTOR) complex (mTORC) 2, a kinase that phosphorylates and activates Akt, an insulin signaling intermediary that regulates glucose and lipid metabolism in adipose tissue, skeletal muscle, and liver. To determine the physiological role of rictor\/mTORC2 in insulin signaling and action in fat cells, we developed fat cell-specific rictor knockout (FRic(-\/-)) mice.<\/div>\n
RESEARCH DESIGN AND METHODS: <\/span>Insulin signaling and glucose and lipid metabolism were studied in FRic(-\/-) fat cells. In vivo glucose metabolism was evaluated by hyperinsulinemic-euglycemic clamp.<\/div>\n
RESULTS: <\/span>Loss of rictor in fat cells prevents insulin-stimulated phosphorylation of Akt at S473, which, in turn, impairs the phosphorylation of downstream targets such as FoxO3a at T32 and AS160 at T642. However, glycogen synthase kinase-3beta phosphorylation at S9 is not affected. The signaling defects in FRic(-\/-) fat cells lead to impaired insulin-stimulated GLUT4 translocation to the plasma membrane and decreased glucose transport. Furthermore, rictor-null fat cells are unable to suppress lipolysis in response to insulin, leading to elevated circulating free fatty acids and glycerol. These metabolic perturbations are likely to account for defects observed at the whole-body level of FRic(-\/-) mice, including glucose intolerance, marked hyperinsulinemia, insulin resistance in skeletal muscle and liver, and hepatic steatosis.<\/div>\n
CONCLUSIONS: <\/span>Rictor\/mTORC2 in fat cells plays an important role in whole-body energy homeostasis by mediating signaling necessary for the regulation of glucose and lipid metabolism in fat cells.<\/div>\n<\/div>\n
<\/div>\n","protected":false},"excerpt":{"rendered":"

Muito j\u00e1 foi publicado acerca da fun\u00e7\u00e3o do complexo mTORC1 em diversos tecidos. o mTORC2, em contrapartida, tem sua fun\u00e7\u00e3o ainda pouco conhecida. Kumar e colaboradores, neste trabalho, trazem novas evid\u00eancias \u00e0 tona, e auxiliam a desvendar a complexidade da … Continue lendo →<\/span><\/a><\/p>\n","protected":false},"author":9,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"_uag_custom_page_level_css":"","footnotes":""},"categories":[6,21,34,39],"tags":[],"class_list":["post-94","post","type-post","status-publish","format-standard","hentry","category-bioquimica","category-fisiologia","category-paper","category-sinalizacao-celular"],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"post-thumbnail":false},"uagb_author_info":{"display_name":"Lucas Guimar\u00e3es Ferreira","author_link":"https:\/\/blog.ufes.br\/lucasgf\/?author=9"},"uagb_comment_info":12,"uagb_excerpt":"Muito j\u00e1 foi publicado acerca da fun\u00e7\u00e3o do complexo mTORC1 em diversos tecidos. o mTORC2, em contrapartida, tem sua fun\u00e7\u00e3o ainda pouco conhecida. Kumar e colaboradores, neste trabalho, trazem novas evid\u00eancias \u00e0 tona, e auxiliam a desvendar a complexidade da … Continue lendo →","_links":{"self":[{"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/posts\/94","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=94"}],"version-history":[{"count":0,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=\/wp\/v2\/posts\/94\/revisions"}],"wp:attachment":[{"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=94"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=94"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/blog.ufes.br\/lucasgf\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=94"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}