Arquivo da categoria: protocolos

QUALITY CONTROL – EFFICIENCY CURVES  Here is a list of the criteria we apply in the lab before we accept the data for efficiency from a dilution curve •use PCR baseline subtraction (not curve fitting default option) •set the threshold … Continue lendo →

Western blot troubleshooting tips 1. No signal The primary antibody and the secondary antibody are not compatible.Use secondary antibody that was raised against the species in which the primary was raised (e.g primary is raised in rabbit, use anti-rabbit secondary). … Continue lendo →