QUALITY CONTROL – EFFICIENCY CURVES
Here is a list of the criteria we apply in the lab before we accept the data for efficiency from a dilution curve
- •use PCR baseline subtraction (not curve fitting default option)
- •set the threshold manually to lab standard
- •check all melting curves are OK
- •check slopes are parallel in log view
- •delete samples if multiple dilutions cross line together (usually at dilute end of curve)
- •delete samples if can detect amplification at cycle 10 or earlier
- •make sure there are 5 or more points
- •check that correlation coefficient is more than 0.990
